Table 2. Restriction analysis of complex rearrangements.
Size, kb | EcoRI (4926) | Eco0109I (1165) | BsgI (2698) | BstXI (3118) | BsaI (4162) | ScaI (4581) | XmnI (4700) | |
---|---|---|---|---|---|---|---|---|
pAG32-1 | 5 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
pAG32-10 | 5 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
pAG32-16 | 5.1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
pAG32-24 | 2.5 | 1 | 0 | 0 | 0 | 1 | 1 | 1 |
pAG32-25 | 2.5 | 1 | 0 | 0 | 0 | 1 | 1 | 1 |
pGG32-6 | 3.3 | 1 | 0 | 0 | 1 | 1 | 1 | 1 |
pGG32-8 | 5.3 | 1 | 1 | 1 | 1 | 2 | 1 | 1 |
pGG32-12 | 3.3 | 1 | 0 | 0 | 1 | 1 | 1 | 1 |
pGG32-17 | 3.3 | 1 | 0 | 0 | 1 | 1 | 1 | 1 |
pGG32-24 | 2.5 | 1 | 0 | 0 | 0 | 1 | 1 | 1 |
pGG32-25 | 6.7 | 1 | 1 | 2 | 2 | 2 | 1 | 2 |
pcMyc-3 | 2.5 | 1 | 0 | 0 | 0 | 1 | 1 | 1 |
pcMyc-4 | 5.8 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
pcMyc-8 | 2.5 | 1 | 0 | 0 | 0 | 1 | 1 | 1 |
pcMyc-13 | 2.5 | 1 | 0 | 0 | 0 | 1 | 1 | 1 |
pcMyc-18 | 2.5 | 1 | 0 | 0 | 0 | 1 | 1 | 1 |
pcMyc-23 | 2.5 | 1 | 0 | 0 | 0 | 1 | 1 | 1 |
pcMyc-24 | 4 | 0 | 1 | 1 | 1 | 1 | 0 | 0 |
pcMyc-27 | 6.5 | 1 | 1 | 2 | 2 | 1 | 1 | 1 |
pcMyc-20 | 5 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
Entries indicate the number of bands generated by restriction digestions. 0, Zero fragments indicate deletion of the analyzed region; 1, one restriction fragment indicates wild type; 2, two restriction fragments indicate duplication of the analyzed region.