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Binding of S1 and fragments to RNA. (a) Mobility-shift assay gels for the binding of tmRNA to S1 and R1–R4 are shown. (b) Curves for tmRNA binding by full-length S1 (▪), N–R1 (•), R1–R4 (▴), R1–R3 (♦), N(□), and R1 (○). (c) Curves for Arc-st11 mRNA binding. Binding data were fit as described in Materials and Methods.
