Fig. 3.
Effects of overexpression of S1 variants on cell growth and translation. Growth curves before (a) and after (b) arabinose induction at 120 min. Strains contained the vector plasmid (▪) or plasmids encoding the full-length S1 (•), N(▴), N–R1 (♦), R1–R4 (□), and R2–R4 (○) proteins. A portion of each culture was pulse-labeled with [35S]methionine at 120 or 180 min and analyzed by SDS/PAGE and autoradiography. The bands corresponding to the overexpressed S1 variants are highlighted with dots.