Skip to main content
. 2016 Dec 1;17(12):2002. doi: 10.3390/ijms17122002

Figure 3.

Figure 3

The effects of Aire on gene expression downstream of TLR3 in GFP-Aire/DC. (A) The IL-12 p40, IL-23, IL-27 p28, and IFN-β transcript expression levels in GFP-Aire/DC and GFP/DC cells were detected by RT-qPCR. All qPCR data are shown as the expression relative to that of GAPDH and are depicted as fold-change relative to the expression in GFP/DC cells, which was normalized to 1; and (B) The IL-27 p28 and IFN-β protein concentrations in GFP-Aire/DC and GFP/DC cells were analyzed by ELISA. Data are shown as the means ± SD from three to six independent experiments. * p < 0.05 and ** p < 0.01 compared with the GFP/DC control; # p < 0.05 compared with the GFP-Aire/DC control.