Table 1.
The effects of Aire on the Poly (I:C)-stimulated phenotypic characteristics of DC2.4 cells.
| MFI % | GFP/DC | GFP-Aire/DC | ||
|---|---|---|---|---|
| Control | Poly (I:C) | Control | Poly (I:C) | |
| CD40 | 0.50 ± 0.25 | 2.60 ± 0.18 1 | 0.64 ± 0.22 | 0.98 ± 0.27 2,3 |
| 1.67 ± 0.64 | 8.35 ± 0.35 2 | 6.61 ± 0.13 | 7.34 ± 1.02 | |
| CD80 | 51.60 ± 12.16 | 69.60 ± 0.64 | 26.20 ± 8.76 2 | 14.51 ± 10.57 3 |
| 30.43 ± 7.36 | 43.67 ± 1.37 | 25.99 ± 0.02 | 8.08 ± 4.57 4 | |
| CD83 | 17.07 ± 0.49 | 8.46 ± 0.85 1 | 13.60 ± 1.70 | 4.08 ± 3.29 3 |
| 13.75 ± 3.17 | 13.18 ± 9.03 | 11.75 ± 1.27 | 9.02 ± 3.89 | |
| CD11C | 7.93 ± 0.49 | 11.70 ± 0.28 2 | 6.69 ± 0.22 | 5.03 ± 0.18 4 |
| 15.59 ± 5.51 | 19.60 ± 0.87 | 17.41 ± 2.75 | 16.14 ± 0.20 | |
| CD86 | 1.82 ± 0.39 | 2.70 ± 1.00 2 | 2.89 ± 0.25 2 | 2.34 ± 0.19 2 |
| 4.03 ± 0.38 | 5.88 ± 1.75 2 | 3.59 ± 0.05 2 | 6.60 ± 0.68 | |
| MHC II | 0.29 ± 0.14 | 1.09 ± 0.11 2 | 0.42 ± 0.29 | 0.42 ± 0.16 3 |
| 0.62 ± 0.41 | 1.37 ± 0.43 | 2.42 ± 0.01 1 | 2.22 ± 1.93 | |
GFP/DC and GFP-Aire/DC were treated with Poly (I:C) for 48 h as described in the Methods. Subsequently, the phenotypic characteristics of GFP/DC or GFP-Aire/DC in the presence or absence of Poly (I:C) treatment were determined by FCM. The results are shown as the means ± SD from three to six independent experiments. %, percentage of positive cells; MFI, mean fluorescence intensity. 1 p < 0.01 and 2 p < 0.05 compared with control GFP/DC; 3 p < 0.05 and 4 p < 0.01 compared with Poly (I:C)-treated GFP/DC.