. 2016 Dec 5;17(12):2035. doi: 10.3390/ijms17122035

Table 1.

Overview of the study protocol. Fasting blood samples are obtained and handled according to the reference protocol or one of eight variant protocols.

Protocol	Processing					Freezing & Storage
	Delay of Incubation	Temperature of Incubation	Centrifugation Parameters			Delay between Sample Preparation & Freezing at −80 °C	Time at −80 °C
	Delay of Incubation	Temperature of Incubation	Speed	Temperature	Time	Delay between Sample Preparation & Freezing at −80 °C	Time at −80 °C
Reference (Ref)	1 h	22 °C	2000 g	20 °C	10′	15′	3 months
Variant 1 (Vp1)	1 h	4 °C	2000 g	20 °C	10′	15′	3 months
Variant 2 (Vp2)	6 h	4 °C	2000 g	20 °C	10′	15′	3 months
Variant 3 (Vp3)	6 h	22 °C	2000 g	20 °C	10′	15′	3 months
Variant 4 (Vp4)	1 h	22 °C	2000 g	20 °C	20′	15′	3 months
Variant 5 (Vp5)	1 h	22 °C	2000 g	4 °C	10′	15′	3 months
Variant 6 (Vp6)	1 h	22 °C	3000 g	20 °C	10′	15′	3 months
Variant 7 (Vp7)	1 h	22 °C	2000 g	20 °C	10′	1 h	3 months
Variant 8 (Vp8)	1 h	22 °C	2000 g	20 °C	10′	15′	48 h