Figure 2.

AQP4 expression in the spinal cord. AQP4 was abundantly expressed across the whole spinal cord with markedly higher intensity in the superficial lamina of dorsal horns, verges of ventral horns, glia limitans and surroundings of blood vessels (A); In the gray matter, protoplasmatic astrocytes AQP4 expression represents a polarized pattern with the highest intensity in the astrocytes foot processes encircling capillaries (B,C) and weaker labeling in processes belonging to the tripartite synapse (C); In the white matter, fibrous astrocytes AQP4 expression is observed along the cell membrane (D,E); Illustrations of AQP4 expression in the spinal cord (C,E) markings in green illustrate AQP4. The blue signal in panels B and D represents DAPI staining of the nuclei. Immunoperoxidase staining of AQP4 (A) and immunofluorescence images (B,D) are modified from Oklinski et al. [21]. AQP4, aquaporin 4; GS, glutamate synthase.