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. 2016 Dec 9;17(12):2058. doi: 10.3390/ijms17122058

Figure 5.

Figure 5

Low-level MeHg induces genes regulating mitochondrial biogenesis via miR-25. (a) qPCR analysis of mature miR-30d, miR-1285, and miR-25 expression levels performed on RNA extracted from ihNPCs treated with 0 nM, 10 nM and 50 nM MeHg; (b) A representative Western blot of p53 expression in ihNPCs. Cells were transfected with 50 nM of miR-25 mimics, miR-mimic negative control#1, miR-25 inhibitor and miR-inhibitor negative control#1 for 48 h; (c) Western blot analysis of p53 expression performed in 10 nM-treated ihNPCs after transient transfection of miR-25 mimics, miR-mimic negative control#1, miR-25 inhibitor and miR-inhibitor negative control#1. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) protein expression has been used as a loading control and a representative figure of three independent experiments has been shown. Results of gray value analysis are also reported (left part); (d–f) qPCR analysis of TFAM, PCG-α and p53R2 expression performed in 10 nM-treated ihNPCs after transient transfection of miR-25 mimics, miR-mimic negative control#1, miR-25 inhibitor and miR-inhibitor negative control#1. All experiments were repeated three times (technical triplicates) with biological triplicates (n = 3). Bar graphs show mean ± SEM (* p < 0.05).