Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Dec 8;17(12):2060. doi: 10.3390/ijms17122060

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Western blot analysis of pErk and Bcl-xL in different human pancreatic cancer cells after treatment with TLR ligands: BxPC-3 (A); MIAPaCa-2 (B); and PaCaDD135 cells (C) were incubated with ODN, LTA, LPS, HMGB1, ODN + HMGB1, LTA + HMGB1, and LPS + HMGB1 and analyzed 6 h (pErk) and 8 h (Bcl-xL) after stimulation. Cofilin (pErk) and β-actin (Bcl-xL) probes were used as controls for protein loading. Relative optical density (ROD) was determined using ImageJ software. Values for proteins of interest were calculated in relation to values of loading controls. Untreated cells were standardized to baseline.

Western blot analysis of pErk and Bcl-xL in different human pancreatic cancer cells after treatment with TLR ligands: BxPC-3 (A); MIAPaCa-2 (B); and PaCaDD135 cells (C) were incubated with ODN, LTA, LPS, HMGB1, ODN + HMGB1, LTA + HMGB1, and LPS + HMGB1 and analyzed 6 h (pErk) and 8 h (Bcl-xL) after stimulation. Cofilin (pErk) and β-actin (Bcl-xL) probes were used as controls for protein loading. Relative optical density (ROD) was determined using ImageJ software. Values for proteins of interest were calculated in relation to values of loading controls. Untreated cells were standardized to baseline.