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ATP proliferation assay of pancreatic cancer cell lines treated with TLR ligands. BxPC-3, MIAPaCa-2, and PaCaDD135 cells were incubated with: (A) LTA and LTA + HMGB1 (TLR2 activation); (B) LPS and LPS + HMGB1 (TLR4 activation); and (C) ODN and ODN + HMGB1 (TLR9 activation). Untreated cells were standardized to baseline. * p < 0.05, ** p < 0.005, *** p < 0.0001, n.s. not significant.
