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Analysis of HK1b-CP/HPts interactions in Bimolecular Fluorescent Complementation (BiFC) assays. Cells were co-transformed using a combination of plasmids expressing HK1b-CP/HPt2 (C,D); HK1b-CP/HPt7 (E,F); HK1b-CP/HPt8 (G,H); HK1b-CP/HPt9 (I,J) and HK1b-CP/HPt10 (K,L) as indicated. As a negative control, the combination of plasmids expressing HK1b-CP/YFPC (A,B) was used. The morphology was observed by differential interference contrast (DIC) microscopy. Scale bar = 10 µm.
