Figure 7.

Functional complementation assay. (A) Different constructs were introduced into the osmodeficient strain (MH179) as indicated and spotted onto different media at a density corresponding to an optical density (OD)₆₀₀ = 1 and three 10-fold dilutions as indicated. The resulting transformants were spotted onto SG-Ura medium for growth control (B) and onto SD-Ura medium for growth rescue (C). Finally, transformants were spotted onto SD-Ura+NaCl media with increasing concentration of NaCl for the osmotic tolerance test (D).