Table 2.
Primer sequences used for real-time PCR.
| Gene in Mice | Sequence of Selected Primer Pairs | Gene in Humans | Sequence of Selected Primer Pairs |
|---|---|---|---|
| Vegf | F: GCAGACTATTCAGCGGACTCA | VEGF | F: AGGGCAGAATCATCACGAAGT |
| R: GGGAGTGAAGAACCAACCTCC | R: AGGGTCTCGATTGGATGGCA | ||
| MMP-1 | F: CCTCGTTGGACCAAAACACA | ADAMTS-5 | F: GAACATCGACCAACTCTACTCCG |
| R: GCGATGGCATCTTCCACAA | R: CAATGCCCACCGAACCATCT | ||
| MMP-3 | F: TCCTGATGTTGGTGGCTTCA | IL-1β | F: CTCCGGGACTCACAGCAAAA |
| R: CACACTCTGTCTTGGCAAATCC | R: GCCCAAGGCCACAGGTATT | ||
| MMP-13 | F: TCACCTGATTCTTGCGTGCTA | IL-6 | F: CCTGAACCTTCCAAAGATGGC |
| R: CAGATGGACCCCATGTTTGC | R: TTCACCAGGCAAGTCTCCTCA | ||
| Adamts-5 | F: GCTGCTGGTAGCATCGTTACTG | RANKL | F: CAACATATCGTTGGATCACAGCA |
| R: GAGTGTAGCGCGCATGCTT | R: GACAGACTCACTTTATGGGAACC | ||
| Il-1β | F: GCTTCCTTGTGCAAGTGTCTGA | HIF-1α | F: GAACGTCGAAAAGAAAAGTCTCG |
| R: TCAAAAGGTGGCATTTCACAGT | R: CCTTATCAAGATGCGAACTCACA | ||
| Tnf-α | F: AGGGATGAGAAGTTCCCAAATG | ACAN | F: GTGCCTATCAGGA |
| R: GGCTTGTCACTCGAATTTTGAGA | R: GATGCCTTTCACCACGACTTC | ||
| Il-6 | F: TCGGAGGCTTAATTACACATGTTC | MMP-1 | F: TCCACAAATGGTGGGTACAA |
| R: TGCCATTGCACAACTCTTTTCT | R: GGTGACACCAGTGACTGCAC | ||
| Rankl | F: AGGCTCATGGTTGGATGTGG | MMP-13 | F: ACTGAGAGGCTCCGAGAAATG |
| R: GCATTGATGGTGAGGTGTGC | R: GAACCCCGCATCTTGGCTT | ||
| Hif-1α | F: GGATGAGTTCTGAACGTCGAAAAG | TNF-α | F: CGAACATCCAACCTTCCCAAAC |
| R: GTGGCAACTGATGAGCAAGC | R: TGGTGGTCTTGTTGCTTAAAGTTC | ||
| Col2a1 | F: AGCAGGAATTCGGTGTGGAC | MMP-3 | F: TCACAGTTGGAGTTTGACCC |
| R: GACATCAGGTCAGGTCAGCC | R: AAGTGCCCATATTGTGCCTTC | ||
| Acan | F: TGGGATCTACCGCTGTGAAGT | COL2a1 | F: AGCAGGAATTCGGTGTGGAC |
| R: CTCGTCCTTGTCACCATAGCAA | R: GACATCAGGTCAGGTCAGCC | ||
| Glyceraldehyde 3-phosphate dehydrogenase (Gapdh) | F: AGGTCGGTGTGAACGGATTTG | GAPDH | F: CATGGAGAAGGCTGGGGCTCATTTG |
| R: TGTAGACCATGTAGTTGAGGTCA | R: GGGGTGCTAAGCAGTTGGTGGT |