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. 2016 Dec 12;17(12):2088. doi: 10.3390/ijms17122088

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Gal-8_1-186 induces chicken erythrocyte agglutination assay. (A) Gal-8_1-186 induce agglutination of chicken erythrocytes with a MAC (Minimum Agglutination Concentration) value of 0.16 µg/mL. “−”represents the negative control well containing no protein; (B) the agglutination capability of Gal-8_1-186 was inhibited by lactose. 0.16 µg/mL Gal-8_1-186 was used in this assay. The MIC (Minimum Inhibition Concentration) of lactose is 0.63 mg/mL. “−”represents the negative control well containing no protein and lactose; “+”represents the positive control well containing no lactose; (C) the agglutination capability of Gal-8_1-186 was inhibited by glycerol. 0.16 µg/mL Gal-8_1-186 was used in this assay. Glycerol cannot inhibit the agglutination activity of Gal-8_1-186 even at 50% (v/v) concentrations. “−” represents the negative control well containing no protein and glycerol; “+” represents the positive control well containing no glycerol.