Figure 2.

SDS-PAGEs of protein crystals. (A) “M” indicates protein molecular marker; “1” indicates the crystallized protein in the first crystallization condition containing 20% (w/v) PEG2000 MME. The molecular weight of this protein is approximately 14 kDa; (B) “M” indicates protein molecular marker; “1” indicates the crystallized protein in the second crystallization condition containing 0.1 M Tris pH 8.5, 0.01 M NiCl₂, 20% (w/v) PEG2000 MME; “2” indicates the protein after his-tag cut off. The protein bands in “1” and ”2” are same that means Gal-8 N-terminal CRD with the linker was crystallized.