Figure 3.

Crystal structures of Gal-8_1-186. (A) The N-terminal tail interacts with the bottom of Gal-8 linker (structure 1); (B) Phe19 could interact with Ile12 through van der Waals forces (structure 1: yellow; structure 2: purple; structure 3: pink; structure 4: cyan; structure 5: green); (C) Ni²⁺ was found to form a complex with Asp25 and two histidine residues (His38 and His147) from another Gal-8N molecule. Two Gal-8 N-terminal CRDs from two asymmetric units were labeled by green and cyan. This figure was generated by using structure 4 (PDB: 5GZF). One pink ball indicates Ni²⁺. Three green balls indicate water molecules. N-terminal and C-terminal of the structure are close to each other and are labeled by gold. C-terminal of the structure is also the starting point of the linker.