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. 2016 Dec 16;17(12):2116. doi: 10.3390/ijms17122116

Figure 5.

Figure 5

Both KF and SG showed potent inhibitory activities on bone resorption in RANKL-induced RAW264.7 osteoclastic cells. (a) The fluosrescence intensity of medium in Fluoresceinamine-labeled chondroitin polysulfate (FACPS)/calcium phosphate (Ca-P)-coated wells after six days of RANKL induction; (b) The resorption pit areas. Areas of the formed pit was analyzed and calculated by software Image-Pro-Plus 6.0; (c) Representative micrographs of bone resorption pits from each group in day 6 was shown. Control (−) represents for group which was treated with only 1% ethanol for six days; Control (+) stands for group in which cells were induced with 1% ethanol, 100 ng/mL RANKL and 30 ng/mL M-CSF for six days; 17-β Estradiol (E2, 10−7M), KF (5, 12.5 μM), and SG (5, 12.5μM) were treatment groups in which induced cells (by 100 ng/mL RANKL and 30 ng/mL M-CSF.) were treated for three days. Results were obtained from two independent experiments in triplicate and expressed as mean ± SEM (Standard error of the mean). ### p < 0.001 versus Control (−); * p < 0.05, ** p < 0.01, *** p < 0.001 versus Control (+).