Figure 1.

The mRNA and protein levels of ELMO3 in primary colorectal cancer (CRC) tissues and adjacent normal tissues: (A) Real-time quantitative PCR was used to detect the ELMO3 mRNA expression level in primary tumors (T) and matched adjacent normal tissues (N) in CRC patients. LNM (+): patients with lymph node metastasis; LNM (−): patients without lymph node metastasis. β-actin was used as a reference gene to normalize ELMO3 expression. The 2^–ΔΔCt method was used to calculate the relative expression ratio. Error bars indicate standard deviation (SD) and (B) Western blot analysis was used to determined protein level of ELMO3. The statistical analysis was performed by Analysis of variance (ANOVA). * p < 0.05, ** p < 0.01.