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. 2016 Dec 17;17(12):2129. doi: 10.3390/ijms17122129

Figure 1.

Figure 1

mRNA and protein expression of human SOD1 and mouse SOD1 in NSC34 cells. (A) The mRNA expression of human SOD1 (hSOD1) was detected by qRT-PCR in wild type (WT) SOD1 NSC34 cells and mutant SOD1 (mtSOD1) NSC34 cells, but was not detectable in empty vector (EV) NSC34 cells; (B) The mRNA level of mouse SOD1 (mSOD1) was detected by qRT-PCR in WT SOD1 NSC34 cells, mtSOD1 NSC34 cells, and EV NSC34 cells; (C) hSOD1 protein expression was measured by Western blot in WT SOD1 NSC34 cells, mtSOD1 NSC34 cells, and EV NSC34 cells. ** p < 0.01 vs. EV group, *** p < 0.001 vs. EV group. n = 3 independent batches of cells for each group.