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. 2016 Dec 17;17(12):2129. doi: 10.3390/ijms17122129

Figure 2.

Figure 2

Homer1b/c expression increased in mtSOD1 NSC34 cells. (A) Homer1b/c protein was detected by immunofluorescence assay in cytoplasm in both wild type (WT) SOD1 NSC34 cells and SOD1 G93A (mtSOD1) NSC34 cells. Scale bar is 25 µm; (B) qRT-PCR detected the mRNA expression of Homer1b/c in WT SOD1 NSC34 and mtSOD1 NSC34 cells; (C) Homer1b/c protein expression level was measured by Western blot in WT SOD1 NSC34 cells and mtSOD1 NSC34 cells; (D) TUNEL staining of NSC34 cells transfected with empty vector (EV), WT SOD1, and mtSOD1. Scale bar is 20 µm; (E) MTT assay of NSC34 cells transfected with empty vector (EV), WT SOD1, and mtSOD1. * p < 0.05 vs. WT group, ** p < 0.01 vs. WT group. n = 3 independent batches of cells for each group.