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. 2016 Dec 17;17(12):2129. doi: 10.3390/ijms17122129

Figure 4.

Figure 4

Lithium and valproic acid (VPA) reduced Homer1b/c expression and apoptosis-related genes expression in mtSOD1 NSC34 cells. (A) TUNEL staining of NSC34 (EV, WT SOD1, and mtSOD1) cells after treatment with LiCl + VPA (LiCl 1.0 mM + VPA 0.6 mM) for 72 h. Scale bar is 20 µm; (B) The mRNA level of Homer1b/c was detected by qRT-PCR in mtSOD1 NSC34 cells after treatment with LiCl (1.0 mM), VPA (0.6 mM), and LiCl + VPA (LiCl 1.0 mM + VPA 0.6 mM) for 72 h; (C,D) Western blot detected the protein levels of Homer1b/c and apoptosis- related genes (Bcl-2, Bax) expression in mtSOD1 NSC34 cells after treatment with LiCl (1.0 mM), VPA (0.6 mM), and LiCl + VPA (LiCl 1.0 mM + VPA 0.6 mM) for 72 h, + with treatment of the drug, - without treatment of the drug. ** p < 0.01 vs. control (Ctl) group, *** p < 0.001 vs. Ctl group. n = 3 independent batches of cells for each group.