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. 2016 Dec 18;17(12):2133. doi: 10.3390/ijms17122133

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Expression of key genes of the WNT/β-catenin signaling pathway in MCF-7 shRNA-GLO1 cells. The plot shows the up- or downregulation of mRNA expression relative to mock-transfected control cells as fold-change calculated by the ΔΔCt method (REST^©2009 Software downloaded from http://www.wzw.tum.de/gene-quantification/) using the Ct values from the genes of interest normalized to the Ct values of GAPDH mRNA (housekeeping gene). Statistical significance was determined by Student’s t-test with: n = 3 and * p < 0.05.