Fig. 1.
T cell activation is CD1d-restricted and antigen (Ag)-dependent. (a and b) Proliferation of ABd T cells stimulated with DC and antigen mixtures was measured in the presence of mAb against CD1a, CD1b, CD1c, CD1d, or isotype-matched control (P3) (20 μg/ml). (c) C1R cells transfected with CD1a, CD1b, CD1c, CD1d, or vector alone (mock) were treated with the antigen mixture at the indicated concentration (in μg/ml). Blocking mAb and polyclonal ABd T cells were added, and proliferation was measured.