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Effect of lactacystin on FoxO1 down-regulation. CEF were serum-starved for 40 h, incubated with lactacystin (10 μM) or LY294002 (20 μM) for 30 min, and then stimulated with PDGF for 15 min. The membrane was probed with anti-FoxO1 Ab or with anti-α-tubulin Ab for a loading control.
