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Tests for focus formation by the FoxO1-Eng constructs. CEF were transfected with 500 ng of DNA constructs by using the dimethyl sulfoxide/polybrene method. The cells were overlaid with nutrient agar for 21 days and then fixed and stained with crystal violet. (A) Empty RCAS vector. (B) RCAS FoxO1-Eng. (C) ASV31(FoxG1). (D) RCAS FoxO1(AA)-Eng. (E) RCAS FoxO1(AA)/H215R-Eng.
