Fig. 3.

IκBα-induced GADD45 expression is essential for apoptosis induction. (A) Apoptosis of prostate cancer cells after transfection with GADD45α, β, and γ. DU145 and PC-3 cells were transfected with GADD45α, β, and γ expression vectors (1μg) and apoptosis was measured after 48 h. Data are means ± SD of three independent transfections for each vector. (B) Apoptosis assay of DU145 prostate cancer cells and MDA-231 breast cancer cells after infection with Ad5CMVIκB or AdCMVβ-gal and infections with lentiviruses encoding GADD45α, β, and γ siRNA duplexes. Data are means ± SD of three independent transfections.