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. 2004 Sep 7;101(37):13618–13623. doi: 10.1073/pnas.0402069101

Fig. 5.

Fig. 5.

Induction of GADD45 expression is essential for JNK activation and apoptosis induction. (A) Apoptosis of prostate and breast cancer cells 48 h after infection with Ad5CMVIκB or AdCMVβ-gal and treatment with the JNK inhibitor JNKII SP600125 (100 nM, Calbiochem). Data are means ± SD of three independent infections. (B) Apoptosis of two prostate cancer cell lines after transfection with expression vectors (1 μg) for the JNK family members, JNK1 and 2, and its upstream regulator MKK4. Data are means ± SD of three independent transfections. (C) Apoptosis of prostate cancer cells after transfection with the GADD45 family members (1 μg) and treatment with the JNK inhibitor, SP600125 (100 nM). Data are means ± SD of three independent transfections. Apoptosis is shown as the percentage of apoptosis for each GADD45 family gene in the absence of the JNK inhibitor. (D) Kinase assay showing inhibition of JNK kinase activity by GADD45 siRNA duplexes (50 nM) in prostate cancer cells infected with Ad5CMVIκB or AdCMVβ-gal.