Table 1. Primers used for real-time PCR.
| Name | GenBank no. | Forward primer | Reverse primer |
|---|---|---|---|
| ACC1 | AF374169 | CCCAGCAGAATAAAGCTACTTTGG | TCCTTTTGTGCAACTAGGAACGT |
| ACC2 | AF290178 | AACTCCCTGCCAAGCTCATG | GGAGGGCCAGGTGTCATTG |
| ACO | NM_017340 | GGCCAACTATGGTGGACATCA | ACCAATCTGGCTGCACGAA |
| CPT-1 | NM_013495 | CCTGGGCATGATTGCAAAG | ACGCCACTCACGATGTTCTTC |
| FAS | XM_126624 | CCTGGATAGCATTCCGAACCT | AGCACATCTCGAAGGCTACACA |
| GPAT | M77003 | ATCTTCAGAACAGCAAAATCGAAA | CAGCGGAAAACTCCAAATCC |
| PPARα | X57638 | CTGCAGAGCAACCATCCAGAT | GCCGAAGGTCCACCATTTT |
| PPARγ | AF156666 | TCAGAGGGACAAGGATTCATGA | CACCAAAGGGCTTCCGCAGGCT |
| SPT | NM_009269 | CCTCCAAGCATCAGGGTTGT | GGATGCAGCCCTCTGTAGCT |
| PGC-Iα | AB025784 | GCGCCAGCCAACACTCA | TGGGTGTGGTTTGCATGGT |
| SREBP-Ic | L16995 | GCAACACTGGCAGAGATCTACGT | TGGCGGGCACTACTTAGGAA |
| CD36 | AF111268 | GGACCATTGGCGATGAGAAA | CCAGGCCCAGGAGCTTTATT |
| MCD | NM_053477 | CAGAGGACCGGCTACGCTAT | CAGCTTACTGATGTGGTGGAAGA |
| SCD-1 | NM_009127 | CCAGAATGACGTGTACGAATGG | GCGTGTGTTTCTGAGAACTTGTG |
| SCD-4 | NM_183216 | GGCTTTCCAGAATGACGTGTATG | GCGTGTGTTTCTGAGAACTTGTG |
| Bax | NM_007528 | CCAAGAAGCTGAGCGAGTGTC | CCTCTGCAGCTCCATATTGCT |
| Bcl2 | NM_009741 | TGGGATGCCTTTGTGGAACT | GAGACAGCCAGGAGAAATCAAAC |
| SIRT1 | NM_019812 | GCCAAACTTTGTTGTAACCCTGTA | TGGTGGCAACTCTGATAAATGAA |
| UCP-1 | U63419 | ACTGGAGGTGTGGCAGTGTTC | TGGGCTTGCATTCTGACCTT |
| UCP-2 | NM_011672 | TGTTGATGTGGTCAAGACGAGAT | CATGGTAAGGGCACAGTGA |
| UCP-3 | NM_009464 | CATCACAAGAAATGCCATTGTCA | TCCAGCAACTTCTCCTTGATGA |
ACO, acyl CoA oxidase; CPT, carnitine palmitoyl transferase; GPAT, glycerophosphate acyl transferase; PPAR, peroxisome proliferator-activated receptor; SPT, serine palmitoyl transferase; PGC, PPARγ coactivator, SREBP, sterol regulatory element-binding protein; UCP, uncoupling protein; FAS, fatty acid synthase; SCD, stearoyl-CoA desaturase.