Fig. 6.

Immunohistochemical detection of HsICAM-1. HsICAM-1 staining in the ventral horn of HsICAM-1 tg mouse (A) and human (C) lumbar spinal cord. Specificity was controlled by staining parallel sections from wild-type mice (B) and by incubating sections of human spinal cord with isotype-matched nonspecific primary antibody (D). HsICAM-1 signal was visualized with diaminobenzidine substrate (brown; arrowheads), and sections were counterstained with hematoxylin (blue). Fluorescent detection of HsICAM-1 (E and F) and NMJ (G and H) in gastrocnemius muscle of tg (E and G) and wild-type (F and H) mice. Two patterns of HsICAM-1 signal appeared in muscle (E): bright staining corresponding to capillary endothelium (arrowheads) and a more delicate signal that colocalized with NMJ (arrows; compare E and G). (Bars = 50 μm.)