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. 2004 Sep 9;101(38):13726–13731. doi: 10.1073/pnas.0405082101

Table 1. Purification of NfdA.

Step Total protein, mg Total activity, units Specific activity, units per mg Yield, %
Cell-free extract 2,430 705 0.29 100
(NH4)2SO4 752 564 0.75 80.0
DEAE-Sephacel 53.2 277 5.2 39.3
Resource ISO 3.81 76.2 20.0 10.8
Resource Q 1.72 49.7 28.9 7.1

Enzyme assay was performed by means of standard assay A.