Table 3. Individual Cys-substituted carboxylase mutants retain activity.
| Carboxylase variant | Carboxylase, pmol/ng·h-1 | Specific activity, % |
|---|---|---|
| Wild type | 9.7 | 100 |
| C99S | 0.2 | 2 |
| C134S | 10.1 | 104 |
| C139S | 11.3 | 116 |
| C288S | 10.9 | 112 |
| C311S | 7.7 | 79 |
| C323S | 9.6 | 99 |
| C343S | 6.1 | 63 |
| C343A | 5.9 | 61 |
| C450S | 1.8 | 19 |
| C598S | 7.4 | 76 |
| C700S | 6.0 | 62 |
| Mock | <0.1 | 0 |
Microsomes from baculoviral- or mock-infected insect cells were solubilized in the absence of propeptide and then assayed for carboxylase specific activity as described in the legend to Table 1.