Table 1. Interactions of NusA AR1–AR2 with λN peptides.

λN peptide	Sequence	Kd, μM	ΔHa, cal/mol	ΔSa, cal/mol per K	Stoichiometry
λN(34-47)	NRPILSLNRKPKSR	3.55 ± 0.11	-9,452 ± 24.01	-27.77	0.965 ± 0.003
λN(31-43)	KPVNRPILSLNRK	9.65 ± 0.23	-13,350 ± 50.92	-22.58	1.263 ± 0.003
N34A	KPVARPILSLNRK	13.48 ± 0.23	12,700 ± 53.09	-21.21	0.862 ± 0.002
R35A	KPVNAPILSLNRK	Below DL	Below DL	Below DL	Below DL
P36A	KPVNRAILSLNRK	12.86 ± 0.24	15,340 ± 56.77	-29.96	1.087 ± 0.003
137A	KPVNRPALSLNRK	Below DL	Below DL	Below DL	Below DL
L38A	KPVNRPIASLNRK	Below DL	Below DL	Below DL	Below DL
S39A	KPVNRPILALNRK	41.24 ± 0.46	-10,650 ± 41.32	-16.25	1.229 ± 0.003
L40A	KPVNRPILSANRK	Below DL	Below DL	Below DL	Below DL

Below DL, below detection limit; A, mutated residue.