Skip to main content
. 2016 Dec 3;8(12):782. doi: 10.3390/nu8120782

Figure 4.

Figure 4

Induction of endothelial junction markers of VE-cadherin, PECAM-1, and ZO-1 by chrysin. The db/db mice were orally supplemented with 10 mg/kg chrysin daily for 10 weeks. The db/m mice were introduced as control animals. Mouse retinal tissue extracts were subject to Western blot analysis with a primary antibody against VE-cadherin and PECAM-1 (A). β-Actin protein was used as an internal control. Bar graphs (mean ± SEM, n = 3) in the right panel represent densitometric results of left blot bands. Values not sharing a common letter differ, p < 0.05. Histological sections of mouse retina were immunohistochemically stained using a primary antibody of ZO-1 (B). The ZO-1 expression was identified as 3,3′-diaminobenzidine staining (brown) and the sections were counter-stained with hematoxyline. Magnification: 200×. Retinal layers are labeled as follows: neurofiber layer/ganglion cell layer (NFL/GCL), inner plexiform layer (IPL), inner nuclear layer (INL), outer plexiform layer (OPL), outer nuclear layer (ONL), and photoreceptor inner segment/outer segment (IS/OS).