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. 2016 Dec 3;8(12):782. doi: 10.3390/nu8120782

Figure 6.

Figure 6

Inhibition of retinal induction of Ang-1, Ang-2, and Tie-2 by chrysin. HRMVEC were cultured in media of 33 mM glucose in the absence and presence of 1–20 μM chrysin for two days (A). Cells were also incubated with 5.5 mM glucose and 27.5 mM mannitol as osmotic controls. The db/db mice were orally supplemented with 10 mg/kg chrysin daily for 10 weeks. The db/m mice were introduced as control animals (B). HRMVEC lysates and mouse retinal tissue extracts were subject Western blot analysis was conducted for the induction of Ang-1, Ang-2, and Tie-2 using a primary antibody against Ang-1, Ang-2, or Tie-2. β-Actin protein was used as an internal control. Bar graphs in the bottom panel represent densitometric results of upper blot bands. Values (means ± SEM, n = 3) not sharing a common letter differ, p < 0.05.