Figure 3.

(A) Levels of proteins associated with WAT inflammation, browning, autophagy, and apoptosis in the inguinal fat of WD and KO mice fed HS or HB diet for 5 weeks; (B) representative picture of immunoblot; (C) TUNEL assay and (D) hematoxylin and eosin (H/E) staining of the inguinal fat. In (A), data are mean ± SEM, n = 8. Results of two-way ANOVA are shown in the tables (D, diet; G, genotype; D × G, interaction; NS, not significant). In (C), the same field is shown stained for total or apoptotic nuclei by DAPI or TUNEL, respectively; in (D), crownlike structure is indicated by arrowheads.