Table 1.
Liver lipid content and acyl-CoA oxidase (ACOX) activity and body fat percentage of wild (WD) and knockout (KO) mice fed high-fat diet composed of soybean oil alone (HS) or with bitter melon seed oil (HB) for 5 weeks 1.
| Liver | Body Fat (%) | |||||
|---|---|---|---|---|---|---|
| Triglyceride (mg) | Cholesterol (mg) | ACOX Activity (nmol/(min·mg Protein)) | Retroperitoneal | Epididymal | Inguinal | |
| WD-HS | 12.2±1.2 b | 1.81 ± 0.19 | 2.62 ± 0.38 b | 0.87 ± 0.11 | 2.16 ± 0.27 | 1.11 ± 0.09 |
| WD-HB | 8.9 ± 1.1 c | 1.63 ± 0.14 | 4.05 ± 0.31 a | 0.39 ± 0.03 | 1.38 ± 0.15 | 0.75 ± 0.07 |
| KO-HS | 12.7 ± 0.7 b | 2.54 ± 0.30 | 1.92 ± 0.24 b | 0.66 ± 0.05 | 1.86 ± 0.09 | 1.12 ± 0.02 |
| KO-HB | 29.5 ± 8.8 a | 2.24 ± 0.17 | 2.46 ± 0.28 b | 0.36 ± 0.04 | 1.28 ± 0.09 | 0.86 ± 0.05 |
| p Values for Two-Way ANOVA | ||||||
| G | <0.01 | <0.0001 | <0.001 | NS | NS | NS |
| D | NS | NS | <0.001 | <0.0001 | <0.01 | <0.01 |
| G × D | <0.05 | NS | <0.05 | NS | NS | NS |
1 Data are means ± SEM, n = 8. Two-way ANOVA was conducted and results are shown in table (D, diet; G, genotype; D × G, interaction; NS, not significant). When there was a significant interaction between D and G, the significance of differences among groups was further analyzed by one-way ANOVA and Duncan’s multiple range test. a–c Values without a common superscript differed (p < 0.05).