Figure 3.

Effect of WPH on spontaneous smooth muscle contractions in the rat distal colon. Examples of raw data recordings from two separate experiments show changes in muscle tension over time for the control, after addition of 1 mg/mL (a) WPH-P; n = 12; (b) WPH-AT; n = 11, and the last 10 min of recovery following a 60 min washout in Krebs buffer; (c) Time-dependence of WPH-AT effect shown as percent change in tension and frequency from the pretreatment control for a 60 min application showing, pooled data from 12 experiments. Treatment with WPC-AT was applied for 60 min (n = 11). Data show mean ± SEM. Asterisks indicate the significance of each treatment relative to controls (** p < 0.01, *** p < 0.001).