Figure 2. PC12 cells transfected with full-length expanded ATX3 (Q84), treated with different concentrations of lithium carbonate and analyzed after 48 h. A, Relative cell viability by the MTT assay. B, Percentage of apoptotic cells analyzed by flow cytometry after Annexin V and propidium iodide staining. C, ATX3-Q84 monomer/aggregate ratio analyzed by western blotting. D, Band densitometry was calculated with ImageJ software, and data of monomer/aggregate ratio were plotted in a graph. All experiments were performed in triplicates and data are reported as means±SE. *P<0.05, Kruskal-Wallis test.
