Figure 3. PC12 cells transfected with full-length expanded ATX3 (Q84), treated with different concentrations of coenzyme Q10 (CoQ10) and analyzed after 48 h. A, Relative cell viability was measured by the MTT assay. B, Percentage of apoptotic cells analyzed by flow cytometry after Annexin V and propidium iodide staining. C, ATX3-Q84 monomer/aggregate ratio analyzed by western blotting. D, Band densitometry was calculated with ImageJ software and the data of monomer/aggregate ratio were plotted in a graph. All experiments were performed in triplicate and data are reported as means±SE. *P<0.05, Kruskal-Wallis test.
