Table 1. Experimental inoculation of vertebrate hosts with Heartland virus*.
| Experimental animal model | Strain | No. | Age, d/sex | Inoculum dose, PFU | Route of inoculation |
|---|---|---|---|---|---|
| Mouse | C57BL/6 | 15 | 21/ F | 104 | ip |
| Mouse | Ag129 | 15 | 21/M and F | 104 | ip |
| Mouse | Ag129 | 15 | 21/M and F | 103 | ip |
| Mouse | Ag129 | 15 | 21/M and F | 102 | ip |
| Mouse | Ag129 | 15 | 21/M and F | 101 | ip |
| Mouse | Ag129 | 15 | 21/M and F | 100 | ip |
| Mouse† | CD-1 | 10 | 2 | 103 | ic |
| Mouse† | CD-1 | 10 | 17 | 103 | ip |
| Chicken | Leghorn | 3 | Adult/F | 104 | sc |
| Hamster | Syrian golden | 5 | 21/F | 104 | sc |
| Goat | Boer | 2 | Adult/M and F | 104 | sc |
| Rabbit | New Zealand white | 3 | Adult/F | 104 | sc |
| Raccoon | Wild-caught | 6 | Adult/M and F | 104 | sc |
*Ag129, interferon-α/β/γ receptor–deficient; ic, intracranial; ip, intraperitoneal; sc, subcutaneous. †CD-1 mice were also inoculated similarly with Lone Star virus (same dose and route as for Heartland virus).