Table 2. Viremia and antibody responses of animals experimentally inoculated with Heartland virus* .
| Animal | Inoculum dose, PFU | Mean peak titer† | dpi | Mean ELISA titer‡ | ELISA positive, no. (%) | Mean PRNT70 titer‡ | PRNT70 positive, no. (%) |
|---|---|---|---|---|---|---|---|
| Mouse (C57BL/6) | 104 | <1.5 | 42§ | 4.4 (0.6) | 15 (94) | 0.7 (0.6) | 10 (63) |
| Chicken | 104 | <1.5 | 14 | NT | NA | ND | 0 |
| Chicken | 104 | NA | 42§ | 3.0 (0.2) | 3 (100) | ND | 0 |
| Hamster | 104 | <1.5 | 42§ | 4.3 (0.5) | 4 (100) | 1.3 (0.4) | 5 (100) |
| Goat | 105 | <1.5 | 28 | NT | NA | 1.0 | 2 (100) |
| Goat | 105 | NA | 42§ | 3.0 | 2 (100) | 1.3 | 2 (100) |
| Rabbit | 104 | <1.5 | 14 | NT | NA | ND | 0 |
| Rabbit |
104 |
NA |
42§ |
4.1 |
3 (100) |
1.2 (0.2) |
3 (100) |
| Raccoon | 104 | <1.5 | 42 | NT | NA | 0.4 (0.6) | 2 (33) |
*Bold indicates initial (not given a booster immunization) sampling of a pair of animals that were later given a booster immunization. dpi, day postinoculation; NA, not assessed; ND, not detected (no titer <1:10 for any animal); NT, not tested; PRNT70, 70% plaque reduction neutralization test. †Detection limit was 1.5 log10 PFU/mL. ‡log10 reciprocal titer (SD). §Animals were given booster inoculations with 104 PFU of Heartland virus at 28 dpi.