Figure 4. Target pulled-down of SecA from E. coli lysates by Inhibitors conjugates.
E. coli MC4100 whole cell lysates (33 μg) were treated with SecA inhibitor conjugates as indicated, and the pull- down bead samples were washed, run on SDS gels, and analyzed: (A) Coomassie Blue staining. Arrow indicates SecA band. (B) Immunoblots by EcSecA antibodies.