. Author manuscript; available in PMC: 2017 Nov 21.

Published in final edited form as: ChemMedChem. 2016 Oct 18;11(22):2511–2521. doi: 10.1002/cmdc.201600421

Table 5.

Effects of SecA inhibitors in channel activity, protein translocation, and ATPase IC₅₀ (μM).

Assays	Protein	RB	SCA-50	SCA-107	SCA-112
A. Channel activity	EcSecA	0.4	2.3	1.6	1.3
A. Channel activity	PaSecA	0.3	3.0	1.3	1.1

B. Protein translocation	EcSecA	1.0	ND	3.5	ND
B. Protein translocation	PaSecA	1.0	ND	3.0	ND

C. ATPase activity	H⁺-ATPase	60.0	>200^*	>200^*	>200^*
C. ATPase activity	SpuB ATPase	>200^*	>200^*	>200^*	>200^*

(A). Inhibition on ion-channel activity of SecAs in oocytes. SecA of E. coli (EcSecA), and P. aeruginosa (PaSecA), were reconstituted with liposomes, and then were assayed for ion-channel activity in oocytes with various inhibitors for 3 hrs. The recordings were for 1 min. n =30. (B). Inhibition on SecA-liposomes protein translocation activity, (C) Effects against other ATPase. ND: not determined.

Highest concentration tested.