. Author manuscript; available in PMC: 2017 Nov 21.

Published in final edited form as: ChemMedChem. 2016 Oct 18;11(22):2511–2521. doi: 10.1002/cmdc.201600421

Table 7.

Effects of azi-9 mutation of EcSecA on SecA inhibitors

IC₅₀: μM	EcSecA	Ion channel Activity		Protein Translocation
IC₅₀: μM	EcSecA	SCA-50	SCA-107	SCA-50	SCA107
SecA-Liposomes	Wild-type	3.1	1.8	ND	3.5
SecA-Liposomes	L515F	9.3	3.8	ND	11.4

Membranes	Wild-type	2.4	1.1	3.9	2.8
Membranes	L515F	4.7	4.1	11.2	8.2

Effects of azi-9 mutation (L515F) of EcSecA on SecA inhibitors, IC₅₀ (μM). Liposomes and SecA-depleted BA13 membranes were used for ion channel activity, while urea-treated 773 membrane was used for protein translocation. ND: Not determined