Figure 1.

MultiStem does not induce cytotoxic activity in T cells. Freshly isolated responder CD3⁺ T cells were stimulated with either allogeneic-irradiated (30 Gy) peripheral blood mononuclear cells or allogeneic-irradiated (30 Gy) MultiStem (PBMCs and MultiStem were from the same donor) at a stimulator:responder ratio of 1:2 for 7 days. Coculture was followed by an assessment of anti-CD3-redirected cytotoxic activity against murine P815 mastocytoma target cells (A) or alloantigen-specific cytotoxic activity against Epstein-Barr virus-transformed B cells (B) at an effector:target ratio of 10:1 in a standard ⁵¹Cr-release assay. Data are expressed as mean ± SEM percentage of anti-CD3-dependent specific ⁵¹Cr-release (% SR) of five independent experiments with four different T cell donors and three different PBMC/MultiStem donors (donors 1, 2, and 3) (A) and mean ± SEM % ⁵¹Cr-release of three independent experiments with two different T cell donors and two different PBMC/MultiStem/B cell donors (donors 1 and 2) (B). Statistical significance was calculated with the unpaired t test. ∗∗∗, p < .001. Abbreviations: EBV, Epstein-Barr virus; P815, murine P815 mastocytoma target cells; PBMCs, peripheral blood mononuclear cells; SR, specific ⁵¹Cr-release.