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. 2016 Jul 27;5(12):1607–1619. doi: 10.5966/sctm.2016-0030

Figure 2.

Figure 2.

Activated T cells do not lyse allogeneic MultiStem cells. Results of anti-CD3-redirected and alloantigen-specific cytotoxic activity of irradiated (40 Gy) EBV+ B cell-stimulated CD8+ cytotoxic T lymphocytes (CTLs) (stimulator:responder ratio of 1:10 for 7 days) against respectively anti-CD3-coated P815 target cells, MultiStem, or EBV+ B cells at an effector:target ratio of 10:1. MultiStem cells and EBV+ B target cells were from the same donor as the EBV+ B cells used for CTL activation. Target cell killing is expressed as mean ± SEM percentage of 51Cr-release of n independent experiments (P815 targets, n = 6; MultiStem targets, n = 6; EBV+ B targets, n = 3). Four different CTL donors and three different MultiStem/B-cell donors (donors 1, 2, and 3) were used. Statistical significance was calculated with the paired t test. ∗, p < .05; ∗∗∗, p < .001. Abbreviations: EBV, Epstein-Barr virus; P815, murine P815 mastocytoma target cells.