Table 1. Post surgery gene expression analyses by reverse transcription-PCR in central nervous system (CNS) areas and the stomach.

Gene	Primers	Annealing temperature (°C)	CNS areas	Gastric tissue
Ghsr	F:TCTTCGTGGTGGGCATCTCA R:AAGCAGATGGCGAAGTAGCG	51	+
Leprb	F:CCAGTGCATCGCCAGGAAA R:ACAGTGAGCTGGGAATGGGC	55	+
Mc4r	F:GGCTTCACATTAAGAGGATCGCT R:TTTATGGAACTCCATAGCGCC	50	+
Cnr1	F:AGGGTACTTCCCACAGAAATTC R:CGTGAAGGTGCCCAGCGTGA	50	+	+
Ghrl	F:TCTTGAGCCCAGAGCACCAGA R:AGTTGCAGAGGAGGCAGAAGC	50		+
Mboat4	F:CTGGGAGGCTCCCTGTGTTCCT R:AAGTCTGCAGCACGGGCCAG	58		+
Gapdh	F:TCCCTCAAGATTGTCAGCAA R:AGATCCACAACGGATACATT	50	+	+

Table lists the specific primer sets and annealing temperatures used to detect the expression levels of GHSR, LEPRb, MC4R and CB1R receptors of preproghrelin (Ghrl) and ghrelin O-acyltransferase (Mboat4), and of the housekeeping enzyme GAPDH, in tissues indicated by the plus symbol (+). In all experimental groups, the hypothalamus, hippocampus, cerebral cortex, cerebellum, amygdala and olfactory bulbs were isolated according to stereotactic coordinates and individually processed.²⁷ Gastric tissue was harvested from near the gastroesophageal junction of the remnant gastric tube of sleeve gastrectomized animals and from identical areas in the other study groups.