Fig 2. Training and validation simulations. The population of EMT models qualitatively captured TGF−β-induced EMT signaling.

(A-I) The population was generated using JuPOETs and trained using 11 different objective functions (41 data sets) taken from Medici et al. [7]. The model captured the simulated experiments for all cases when compared to randomized controls. (J-L) The model populations were also compared against untrained temporal data to measure the effectiveness as a pure prediction. The western blot data was reproduced from Medici et al. [7]. The intensity of each band was estimated using the ImageJ program. These blot intensities were then used as the training data for the EMT parameter estimation studies.