Figure 4.

Lyn stabilizes AR protein expression. (a) Lyn knockdown reduces AR expression and nuclear translocation. LNCaP cells transfected with 10 nM Lyn or Ctr siRNA in the presence of CSS for 24 h and were treated ±R1881. Immunofluorescence was performed using AR (green) and DAPI (nuclei-blue). (b) Lyn knockdown decreases AR and PSA protein expression. LNCaP were treated with Lyn or Ctr siRNA is a dose-dependent manner. Total proteins were extracted and western blots were performed using Lyn, PSA, AR antibodies and vinculin was used as a loading control. (c) Lyn knockdown abrogates R1881-induced PSA at protein levels. LNCaP and C4-2 were transfected with 10 nM of Lyn and Ctr siRNA and were treated ±R1881. Total proteins were extracted and western blots were performed using Lyn, PSA, AR and vinculin was used as a loading control. (d) Lyn overexpression increases AR and PSA protein expression. Total proteins were extracted from LNCaP^Lyn and LNCaP^Mock cultured in FBS (left panel) or from LNCaP^Lyn and LNCaP^Mock cultured for 24 h in CSS and treated ±R1881 (right panel). Western blots were performed using Lyn, PSA, AR antibodies and vinculin was used as a loading control.