Figure 6.

Lyn is required for CRPC progression in vivo. (a) Stable Lyn knockdown reduces AR expression in vitro. Total proteins from LNCaP^sh-Lyn and LNCaP^sh-Ctr cells were extracted and western blot was performed using AR, PSA and Lyn antibodies and Vinculin was used as a loading control. (b) Lyn knockdown decreases PSA: maximum of PSA was measured in mice bearing LNCaP^sh-Ctr tumors prior to castration and in mice bearing LNCaP^sh-Lyn at 7 weeks (***P<0.0001). (c) Lyn knockdown prolongs time to castration: average time post inoculation to reach serum PSA of 50 ng/ml required for castration represent eight mice/group ±s.e.m. (***P<0.0001). (d) Lyn knockdown does not affect tumor volume pre castration, tumor volume was measured prior to castration. Data represent five mice/group ±s.e.m.